
Acquisition Software Chapter 4 – Setting up a Scan 21
4 Setting up a Scan
This chapter gives a detailed description on how to customize scan settings and how to setup the
system for maximum performance and reliability. The necessary settings encompass the selection of
appropriate microscope, illumination and camera settings, the Autofocus setup, the parameterization of
the well plates and the details of the imaging to be performed at each position.
4.1
Autofocus Settings .......................................................................................22
4.1.1 Coarse Autofocus......................................................................................24
4.1.2 Fine Autofocus ..........................................................................................25
4.1.3 Gradient Autofocus Adjustment: Step-by-step..........................................25
4.1.4 Autofocus with Interpolated Starting Positions: Step-by-step ...................27
4.1.5 Object-based Autofocus............................................................................28
4.2 Acquisition Settings......................................................................................30
4.3 Live Settings.................................................................................................33
4.4 Plate Manager..............................................................................................35
4.4.1 Well pattern...............................................................................................35
4.4.2 Positions in well.........................................................................................36
4.5 Plate Type Settings......................................................................................38
4.5.1 Well Measurement........................................................................................40