Инструкция OLYMPUS scan R acquisition — страница 24

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18 Chapter 3 – The Main User Interface
Microscope: Filter cube. Select the filter cube to be used. Note that a filter cube switch is time-
consuming even if the system features the fast IX2_FRFACA filter cube turret. Imaging speed can be
increased considerably by using multi-band filter sets instead of several single-band sets.
Microscope: Objective. Select the objective to be used. Note that a switch of objectives during an
experiment is possible but rather time-consuming. In order to maintain the same focus position for
different objectives use the Objective Parfocality setup (see Chapter 6.3,
Objective Parfocality
).
Defined Color Channels. Select predefined color channels. The objective, filter cube, illumination
pathway, excitation filter/ contrast inset and intensity of the illumination are set according to the
settings of the predefined color channel. You can change the settings individually. This is indicated by a
‘*’ behind the name of the color channel. Click on save to store the new settings.
Illumination: Fluorescence / Transmission. Select one of the two possible illumination modes: epi-
fluorescence illumination with the MT20 or transmission illumination.
Illumination: Excitation filter / Contrast insert. Select a MT20 excitation filter for a Fluorescence image
or a transmission condenser contrast insert for a Transmission image, respectively. It has to match with
the Microscope: Filter cube selection.
Illumination: Light intensity. Use the slider to set the illumination intensity.
Focus. Performs an autofocus procedure (see Chapter 4.1,
Autofocus Settings
) according to the
current settings.
Ocular view. Enables the eyepiece. As long as the eyepiece is enabled, the image is not updated and
Ocular view enabled
is shown in the upper left of the image. Click again to disable the eyepiece.
Image display when ocular view is enabled.
3.4.2 Z Focus
Z focus: Slider. Use the slider for larger movements of the microscope Z-drive.
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