Инструкция OLYMPUS scan R acquisition — страница 28

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Инструкция для OLYMPUS scan R acquisition на русском языке. Документ можно использовать для ознакомления с информацией, относящейся к данной модели.

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22 Chapter 4 – Setting up a Scan

The scan^R Screening Station is a complex system with a number of hardware devices and a wide

range of experimental possibilities. In order to perform a scanning experiment a complete set of

parameters is required with information that allows the controller to synchronize the motorized

microscope modules, the illumination source and the motorized stage with the camera, that configures

the autofocusing procedure and, last not least, defines the type of imaging experiment to be executed

at a precise pattern of positions on the plates and within the wells.

The Edit Scan dialog window allows you to modify and verify all settings. To open it, click the Edit

button or select FileEdit.

All modifications in the settings persist until they are changed again. When starting a scan, the

complete settings will be stored in the resulting directory in a file named

experiment_descriptor.xml

Note that by default the current user settings will be stored in

DefaultSettings.xml

in the in the scan^R

installation folder in the directory .\Acquisition\Scan Settings Library\current user, where current user

corresponds to the Windows login name. This means that when multiple (Windows-) users start the

system, for each user scan^R will load each user’s default settings. Another filename or folder to store

the user settings can be set via FileSave as. It is also possible to reload a saved set of settings via

FileOpen and load an

experiment_descriptor.xml

file or a click on the Open button and change the

settings at will.

The Edit Scan dialog window groups the settings on three tabs:

• Autofocus to define and optimize the autofocus procedure

• Acquisition to define and visually control the microscope, camera and illumination settings, to set

cycle times for scan repetitions and to setup the acquisition of z-stacks

• Plate Manager to choose the well plate format, select the wells and the positions within wells to be

imaged.

4.1 Autofocus Settings

Test Current system settings. Click here to open the Autofocus Settings window. It allows you to

crudely focus and to control if the autofocus channel settings lead to images suitable for autofocusing.

When opening the Autofocus settings window, the Autofocus tab remains active. Move the

Autofocus settings windows aside to access the Autofocus tab and change the settings

(e.g. exposure time, etc.).

Type. Select the method to quantify the image focus from the following options:

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Инструкция OLYMPUS scan R acquisition — страница 28

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