Инструкция OLYMPUS scan R acquisition — страница 23

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Инструкция для OLYMPUS scan R acquisition на русском языке. Документ можно использовать для ознакомления с информацией, относящейся к данной модели.

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Acquisition Software Chapter 3 – The Main User Interface 17

3.4 Devices – Microscope Control and Live View

The Devices button opens the Device Control dialog that starts live image acquisition and allows to

access a selection of hardware settings directly. The device view can be used as a preview to get an

impression of the sample and to change defined color channels.

3.4.1 Microscope Settings

Camera: Exposure time [ms]. The exposure time determines the period of time during which the CCD

chip is sensitive to incoming light, in other words, during which photons are collected and converted

into charges to be read out afterwards. Enter a value manually or use the Auto set button.

Auto set. Use the auto set button to obtain a value for the exposure time. The exposure time is set such

that the mean intensity of the objects equals a certain value. When the original image was saturated it

may be necessary to press the several times in order to set the exposure time properly. (Note that

when using the Auto set button in order to set the exposure time for different assays, the value set for

the intensity threshold object detection algorithm of the scan^R Analysis software will be the same for

these assays).

Camera: Binning. The CCD chip is composed of many light-sensitive units (pixels). These pixels can be

read out individually (binning = 1x1) or the signal of neighboring pixels can be combined electronically

during data readout (binning > 1x1). Binning reduces the spatial resolution but increases the sensitivity

and thus reduces the exposure time required for a good signal-to-noise ratio. It further reduces the

amount of data and consequently increases the readout speed. Therefore binning is recommended if

weak signals have to be detected at high acquisition rates or if spatial resolution is of minor

importance.

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Инструкция OLYMPUS scan R acquisition — страница 23

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