Инструкция OLYMPUS LV200 — страница 2

Инструкция

PDF

0,03 МБ

Телескопы / микроскопы / бинокли

МилаДок395

Инструкция для OLYMPUS LV200 на русском языке. Документ можно использовать для ознакомления с информацией, относящейся к данной модели.

Инструкция представлена в формате PDF, просмотр, скачивание на компьютер, смартфон бесплатно. Возможность печати зависит от используемой программы и настроек устройства. Содержание, структура и объём документа могут различаться в зависимости от модели, версии и редакции руководства. Перед использованием рекомендуется убедиться, что инструкция соответствует вашей модели.

change the gain in live mode to the needed value.

Experiments:

1) The customer has to know the difference between fluorescence and luminescence.

Signals are much weaker (factor 1000 to 1 000 000 !!!)

2) Highest intensity with low magnifications!

3) Due to 0.2 tube lens magnifications of a 20x lens means 4x in reality!

4) Use always glass bottom dishes for higher magnifications.

5) The sample has to be completely stable. During the long integration times movements

or vibrations will lead to a complete loss of the signal.

6) Please tell customer not to open light source or changing filter wheel (blocker) during

long exposure.

This can damage the camera. Keep the intensity of light source at the minimal position.

7) Some luminescent sources need oxygen. They will not work under a cover slip!

8) Focus: The focus of transmission and luminescence is not the same!

9) Focus with transmission (change position of upper filter wheel using hand switch

until you see the light.

10) Reduce light to a minimum (lowest aperture and energy) !!

11) The luminescence focus is just between the transmission focus “ black granules in

cells” to “glass like appearance”

12) In most cases you will not see live luminescence!

13) For luminescence switch upper filter position to the next position which includes

blocker or use

PC control (condenser)

The expectations of the customer will be sometimes unrealistic. They will compare it with

fluorescence

and confocal without thinking. Always ask them if they ever have seen a luminescence image!

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Инструкция OLYMPUS LV200 — страница 2

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