Инструкция OLYMPUS LV200

Инструкция

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Инструкция для OLYMPUS LV200 на русском языке. Документ можно использовать для ознакомления с информацией, относящейся к данной модели.

Инструкция представлена в формате PDF, просмотр, скачивание на компьютер, смартфон бесплатно. Возможность печати зависит от используемой программы и настроек устройства. Содержание, структура и объём документа могут различаться в зависимости от модели, версии и редакции руководства. Перед использованием рекомендуется убедиться, что инструкция соответствует вашей модели.

LV200 Installation:

1) Place the system in a dark room if possible or avoid direct strong light!

2) Install system table.

3) Place LV200 onto table right above the prepared whole.

4) Don’t place the PC, controllers onto the table (vibrations)

5) Connect transmitted light source

6) If you want to use filter wheels in cellM connect UCB with PC

7) The filter wheels are marked at the cable with FW1 and FW2. Connect them in the vice

versa

order into UCB ( FW1 in 2. and FW2 in 1.) !

Choose “IX/51/71” in OBS config

Choose “Contrast inserts” for upper filter wheel including blockers

Choose “Filter Cubes” for lower filter wheel (empty in standard configuration)

8) Connect cables for temperature control (connection cable at the back of LV200)

9) Screw in the tube lens carefully from the bottom

10) Camera installation:

a) Fix the c-mount adaptor onto the camera

b) Remove the small black metal part at the front to get access to the mounting

screw.

c) Use the long hex wrenge 3 (small whole at the front) to fix the camera. Caution

the camera may fall down !!

11) The camera is used without a trigger cable to avoid heating of the chip

12) To change the objectives you have to loose the screws for the incubation chamber.

During a

demo please leave this screws loose, to have the possibility of changing the objective

13) You can only use 35 mm Petri dishes (and with some limitations also slides). Best glass

bottom Petri dishes

Important points for Software: only for cellM (until now not possible with Andor

iKonM)

1) The integration times will be much higher as in fluorescence may be 100 000 ms or

even 1 000 000 ms.

2) The 100 s limit of integration time is solved from version 3.1

3) In the standard configuration there are light blockers in every second position of the

filter wheel. In bioluminescence mode use them to prevent light passing the light

guide. Turn off light source during long experiments. Lowest energy level and almost

closed shutter at the light source gives

enough light.

4) Bright field. Change light blockers by hand switch or PC after you have decreased the

integration time lower than 50 ms.

5) Acquisition using experiment manager:

don’t use trigger cable. The chip will get too warm.

only use “simple acquisition” instead of image type button

use “non synchronous” mode

ad a time loop around the acquisition (is needed for nonsynchronous mode)

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Инструкция OLYMPUS LV200

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