
Software Manual Contents
Contents
Chapter 1
Imaging stations for life
science experiments
Chapter 2
A system chart and a list
of all components
Chapter 3
Getting you started – a
quick guide through the
main features of the
imaging stations and how
to use them
Chapter 4
Simple ways to take
images, how to control
the hardware modules
and which parameters to
set
Chapter 5
Beyond snapshots:
setting up simple and
complex experiments in
an intuitive, graphical
way
1 Introduction .................................................................................. 1
2
System Overview..........................................................................
2.1 System Chart....................................................................................4
2.2 Hardware ..........................................................................................5
2.2.1 Motorized Microscope Modules....................................................... 5
2.3
Software ...........................................................................................6
3 Brief Introduction to the Software and First Steps...................
3.1 The User Interface .........................................................8
3.1.1 The Image Manager..........................................................................
3.1.2 The Viewport Manager ................................................................... 1
3.1.3 The Viewport................................................................................... 1
3.2 Simple Image Acquisition ...............................................................12
3.3 Saving Images – The Database......................................................13
3.4 Loading Images ..............................................................................14
3.5 Conducting Experiments with the Experiment Manager................14
3.6 Displaying Multi-Color Images .......................................................16
3.7 Displaying Sequences ....................................................................17
4 Image Acquisition and Hardware Control .............................. 1
4.1 Simple Image Acquisition ...............................................................20
4.1.1 Snapshot and Live View ................................................................. 2
4.1.2 AVI Recorder .................................................................................. 21
4.2
Camera Control ..............................................................................21
4.3 Illumination Control.........................................................................25
4.4 Microscope Control........................................................................26
4.5 Motorized Stage Control ................................................................29
4.5.1 Defining a Positions List ................................................................. 2
4.5.2 Correcting a Positions List ............................................................. 3
4.5.3 Calibrating the Motorized Stage..................................................... 32
4.6
Autofocus .......................................................................................34
4.6.1 Autofocus options…....................................................................... 35
4.6.2
Executing an Autofocus Scan ........................................................ 3
5 Experiment Manager.................................................................. 3
5.1 A Graphical Tool.............................................................................38
5.2 Concept of Usage ..........................................................................39
5.2.1 Experiment Manager Components ................................................ 3
5.2.2 Arrangement and Customization.................................................... 4
5.3 Setting Up Experiment Plans .........................................................42
5.3.1 Types of Graphical Icons and the General Principles of Usage..... 42
5.3.2
The Command Symbols and Their Properties Pages .................... 43