Инструкция OLYMPUS xcellence — страница 29

Инструкция

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Инструкция для OLYMPUS xcellence на русском языке. Документ можно использовать для ознакомления с информацией, относящейся к данной модели.

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Software Manual Chapter 3 – Brief Introduction and First Steps 13

– The camera starts acquiring images at maximal speed. None is stored; instead, the

newest image overwrites the previous one in the temporary buffer.

– The current image is displayed in the active Viewport.

– A single image icon appears in the Image Buffer Box.

8. Focus your sample with the microscope Z-drive. (Even if the optional PIFOC is available

for focus change, its total range may be too narrow to find the focus at the beginning.

For this reason it is usually better to start with the Z-drive.)

9. If necessary, change the Binning factor in the Camera Control window: 1x1 in order to

get highest spatial resolution, larger factors increase the signal intensity at the cost of

resolution.

10. Adjust the Exposure time for a good signal-to-noise ratio.

11. Click Snapshot in the Acquisition toolbar or the Camera Control window to stop the

Live View. The very last image is being stored as a Snapshot in the Image Buffer Box

and displayed in the Viewport.

12. Click Shutter in the MT20 / MT10 control box to stop the illumination.

13. Save your image as described in the next chapter.

Select an empty buffer in the Image Manager if you want to acquire a new image, oth-

erwise the current image will be overwritten and lost: snapshots are only stored tempo-

rarily and need to be saved.

3.3 Saving Images – The Database

To save a snapshot in the most basic way select FileSave from the menu bar or use the short cut

<Ctrl + s>. The snapshot will be stored in a 16-bit tiff format by default. Other data types can be

chosen as usual in the Save Image As window. As with other files, you have to give a name and

select the destination (path) of the storage.

features a database module for the storage of images and entire experiments including

Experiment Plans, data sets, analyses and so on. The module is explained in detail in Chapter 12,

Database.

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Инструкция OLYMPUS xcellence — страница 29

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