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Инструкция OLYMPUS xcellence — страница 13

Инструкция ФорматPDF Размер7,12 МБ Страниц402 КатегорияТелескопы / микроскопы / бинокли ДобавилOlegMaster

Инструкция для OLYMPUS xcellence на русском языке. Документ можно использовать для ознакомления с информацией, относящейся к данной модели.

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Software Manual Contents
Chapter 10
Sophisticated analyses of
fluorescence intensities,
mostly for time se-
quences and Z-stacks
9.4.2 Create Measurement Sheet.......................................................... 203
9.4.3
Deleting Measurement Results..................................................... 20
4
9.4.4 Image Link .................................................................................... 205
9.4.5
Show/Hide Statistic...................................................................... 205
9.4.6
Select Measurements................................................................... 205
9.4.7
Define Statistics............................................................................ 207
9.4.8
The PreferencesMeasure Tab................................................... 20
8
9.4.9 Measurement Sheets: Statistics................................................... 20
8
10 Intensity Analyses ....................................................................211
10.1 Pixel Value ....................................................................................212
10.2 Histogram .....................................................................................213
10.3 Line Profiles: Intensity ..................................................................214
10.3.1 Horizontal Line Profile................................................................... 21
4
10.3.2 Vertical Line Profile....................................................................... 215
10.3.3
Arbitrary Line Profile ..................................................................... 215
10.3.4
Average Intensity of Neighboring Pixels....................................... 21
6
10.4 Regions of Interest – ROIs............................................................217
10.4.1 General ......................................................................................... 217
10.4.2
Drawing ROIs................................................................................ 217
10.4.3
ROI Measurements (2-D).............................................................. 22
0
10.5 Background Subtraction... ...........................................................221
10.5.1 General ......................................................................................... 221
10.5.2
Subtracting the Image Background ............................................. 221
10.6
Intensity Kinetics in Time and Z ...................................................223
10.7 DeltaF / F (∆F/F) Analysis .............................................................224
10.7.1 General ......................................................................................... 22
4
10.7.2 Generating a (∆F/F) sequence ...................................................... 22
4
10.8 Ratio Analysis ...............................................................................226
10.8.1 General ......................................................................................... 22
6
10.8.2 Generating a Ratio Sequence ...................................................... 227
10.9
Spectral Unmixing ........................................................................228
10.9.1 Application.................................................................................... 22
8
10.9.2 The Problem ................................................................................. 22
9
10.9.3 The Solution.................................................................................. 23
0
10.9.4 How Does it Work?....................................................................... 231
10.9.5
Spectral Unmixing with .............................................. 232
10.9.6
Calibration .................................................................................... 233
10.9.7
Unmixing....................................................................................... 23
4
10.9.8 Unmixing of Color Camera Images .............................................. 23
6
10.10 Phase Color Coding and Analysis ................................................237
10.10.1 Phase Color Coding ..................................................................... 237
10.10.2
Phase Analysis ............................................................................. 23
8
10.11 Colocalization ...............................................................................238
10.12 The FRET Software Module .........................................................240
10.12.1 Image Acquisition ......................................................................... 24
0
10.12.2 FRET Image Correction Factors................................................... 242
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