
Analysis Software Manual
4.2.3 Color Gating................................................................................... 49
4.3 Well Results..................................................................................... 52
4.3.1 Measurement Results.................................................................... 52
4.3.2 Well Results: Populations.............................................................. 53
4.3.3 Export Definitions........................................................................... 54
4.3.4 Export results of individual objects ................................................ 56
5 Example Assay – Step by Step........................................................... 57
5.1 Setting-up and Executing an Assay................................................. 58
5.2 Analyzing the Data .......................................................................... 61
5.3 Time-Lapse Analysis ....................................................................... 64
6 Appendix.............................................................................................. 71
6.1 File Conversion................................................................................ 72
6.1.1 Images List .................................................................................... 72
6.1.2 Image Channel Definition .............................................................. 73
6.1.3 Scan Settings................................................................................. 74
6.1.4 View Conversion Results............................................................... 74
6.2 FCS Export Functionality................................................................. 75
6.3 Libraries........................................................................................... 77
6.3.1 Object Analyzers Library (OAL)..................................................... 77
6.3.2 Object Finders Library (OFL)......................................................... 78
6.3.3 Image Processing Library.............................................................. 78
6.3.4 Virtual Channel Library .................................................................. 79
6.4 Valid functions for derived parameters............................................ 80