
Acquisition Software Contents
Contents
1 Introduction...............................................................................................1
1.1 Abstract..........................................................................................................2
1.2 Technical Support ..........................................................................................3
1.3 Safety Precautions.........................................................................................3
2 Short Step-by-Step Guide.............................................................................5
3 Main User Interface .....................................................................................11
3.1 Front-Panel Displays....................................................................................12
3.2 Display Context Menu..................................................................................14
3.3 Buttons.........................................................................................................16
3.4 Devices – Microscope Control and Live View..............................................17
3.4.1 Microscope Settings..................................................................................17
3.4.2 Z Focus .....................................................................................................18
3.4.3 XY Navigation ...........................................................................................19
3.5 Menus ..........................................................................................................19
4 Setting up a Scan ........................................................................................21
4.1 Autofocus Settings .......................................................................................22
4.1.1 Coarse Autofocus......................................................................................24
4.1.2 Fine Autofocus ..........................................................................................25
4.1.3 Gradient Autofocus Adjustment: Step-by-step..........................................25
4.1.4 Autofocus with Interpolated Starting Positions: Step-by-step ...................27
4.1.5 Object-based Autofocus............................................................................28
4.2 Acquisition Settings......................................................................................30
4.3 Live Settings.................................................................................................33
4.4 Plate Manager..............................................................................................35
4.4.1 Well pattern...............................................................................................35
4.4.2 Positions in well.........................................................................................36
4.5 Plate Type Settings......................................................................................38
4.5.1 Well Measurement........................................................................................40
5 Running a Scan...........................................................................................43
5.1 Systems with Hardware Autofocus ..............................................................44
5.1.1 Plates with Predefined Starting Position ...................................................44
5.1.2 Slides without Predefined Starting Position ..............................................44
5.2 Systems without Hardware Autofocus .........................................................46
5.2.1 Plates with Predefined Starting Position ...................................................46
5.3 Autofocus Adjustment during a Scan...........................................................47
5.4 Online Image Browsing................................................................................47
5.5 Online Analysis ............................................................................................48